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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Protein Expr Purif. 2007 Dec 15;58(2):229–241. doi: 10.1016/j.pep.2007.11.018

Table 3.

Purification of His8-MBP-fl-cytb5.

Step Volume (mL) Total protein (mg)a Yield of fusion protein (%)
Sonicated cellsb 50 789 100
Cell-free lysate 41 571 72
Ni IMAC 8.5 73c 9.3
a

Determined by Bradford assay [58].

b

Prepared from 7.8 g of E. coli cells obtained from 2 L of IPTG-induced culture medium.

c

Yield of His8-MBP-fl-cytb5 fusion protein. A yield of 18 mg of fl-cytb5 (25% of total protein yield) was present in the heme-reconstituted fusion protein as determined by optical spectroscopy. This measurement was consistent with the 26% contribution of fl-cytb5 to the mass of the fusion protein.