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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Protein Expr Purif. 2007 Dec 15;58(2):229–241. doi: 10.1016/j.pep.2007.11.018

Table 5.

Kinetic parameters for the reduction of cytochrome b5 variants by His8-cytochrome b5 reductase. a

Substrate Vmax, s−1 Km, μM V/K, μM−1 s−1 R
His8-MBP-fl-cytb5b 84 ± 18 5 ± 2 17 ± 7 0.975
His8- cytb5-sdc 25 ± 5 5 ± 2 5 ± 2 0.977
a

Reactions were performed as indicated in Material and Methods.

b

Fusion protein from expression with pVP56K (Fig. 1A).

c

cytb5 soluble domain from expression in pVP55A (Fig. 1C).