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86Rb influx as a function of Na, Rb, and Cl concentration for point mutant GV-LLhNKCC1 and chimera h1r2A0.7. Flux procedures are described in Experimental Procedures. Data from 18 to 24 flux rows were averaged for GV-LL and compared with values for hNKCC1 from Fig. 3 (n = 42–55) and h1r2A0.7 from ref. 16 (n = 18–35).
