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. Author manuscript; available in PMC: 2008 Nov 1.
Published in final edited form as: Biol Blood Marrow Transplant. 2007 Sep 21;13(11):1294–1303. doi: 10.1016/j.bbmt.2007.07.014

FIGURE 1. Deficiency of Sema4D decreases cytokine production of Tcells and DCs and lowers T cell expansion in an allogeneic in vitro setting.

FIGURE 1

BALB/c stimulators and T cells from either B6 wt (black bars, allogeneic), Sema4D −/− (grey bars, allogeneic), or BALB/c Tc (white bars, syngeneic) were used., Fig 1a-c, BALB/c splenocytes irradiated and co-cultured with either BALB/c, B6 wt, or Sema4D −/− Tcells at a 2/1 stimulator/responder ratio. (a)- proliferation (72 hours), (b)- IL-2 production (48 hours), (c)- IFNγ (48 hours). (d-e) TNFα and IL12p70 levels measured from supernatants in 18-24 hours co-cultures with non-irradiated CD11c+ BALB/c DCs and BALB/c T cells (white bar), B6 wt T cells (black bars), Sema4D −/− Tcells (gray bar) at a 2:1 ratio. *p<0.01 **p<0.02