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A mutation in the pat7 NLS sequence in Rad52 does not affect its ability to repair MMS induced DNA damage. A spot assay of rad52Δ strains transformed with plasmids expressing RAD52-YFP rad52-R234A-YFP or rad52-R234A-YFP-NLSSV40 as indicated. A strain transformed with an empty plasmid is also included in the analysis. Serial 10-fold dilutions of each transformed strain were spotted on selective media containing either no or 0.0025% MMS. Pictures were captured after 2 days.
