Table 2.

Biological activities obtained for compounds 22, 25–34. The compounds were assayed as described under Table 1. Compounds 25–27, 29 and 34 have a relatively low water solubility due to their high lipophilicity. This is reflected in this assay by a similar or reduced activity at 25 µM versus 2.5 µM. Concentration-dependent increase in activity however was observed in full concentration response curves, with maximal effects at 10 µM.

Cpd	R	2.5 µM of Cpd, 1 µM of GABA	25 µM of Cpd, 1 µM of GABA	1 µM of GABA
		Effect (%) ± sem	Effect (%) ± sem	pEC50 ± sem	Emax (%) ± sem
22		80 ±1	93 ± 5	6.06 ± 0.05	83 ± 4
25		90 ± 1	80 ± 2	5.56 ± 0.13	132 ± 14
26		125 ± 4	141 ± 6	5.46 ± 0.10	185 ± 15
27		122 ± 3	110 ± 0	5.78 ± .03	183 ± 4
28		82 ± 5	52 ± 4	-	-
29		128 ± 4	70 ± 4	5.78 ± 0.03	137 ± 3
30		28 ± 1	32 ± 2	-	-
31		36 ± 2	69 ± 6	-	-
32		29 ± 1	39 ± 2	-	-
33		40 ± 3	21 ± 7	-	-
34		58 ± 11	59 ± 4	-	-