Fig. 3.

Effect of β-tryptase reactivation on α2M. Inactive monomers of β-tryptase were incubated with α2M in the presence of heparin at pH 6.0 Western blotting analysis was done using rabbit anti-α2M Ab. Lane 1, α2M alone at pH 7.2. Lane 2, trypsin and α2M at pH 7.2. Lane 3, α2M alone at pH 6.0. Lane 4, β-tryptase monomers and α2M at pH 6.0. Reproduced with permission from Biochemistry, 2004, 43, 10757-10764. © 2004 American Chemical Society.