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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Surgery. 2007 Dec 27;143(3):334–342. doi: 10.1016/j.surg.2007.09.039

Fig. 2. Exogenous ghrelin pretreatment inhibits LPS-induced release of IL-1β in a dose-dependent and time-dependent manner through GHS-R in RAW 264.7 macrophages.

Fig. 2

The measurements are expressed in pg/ml units+/-SD and are derived from three independent experiments. Fig.2a shows that LPS treatment stimulates the release of IL-1β significantly above the negative controls (*, p<0.05 vs. no treatment) and ghrelin pretreatment 4 h prior to LPS treatment (1μg/ml) for 24 h inhibits the release of IL-1β (**, p<0.05 vs. LPS alone). Fig.2b shows that ghrelin mediated inhibition of LPS-induced IL-1β release is time dependent being the most pronounced at 24 h after LPS treatment (*p<0.05 vs. LPS treatment alone). Fig 2c shows that GHS-R antagonist D[lys-3]-GHRP-6 abrogates ghrelin-mediated inhibition of IL-1β release as measured at 0-30h. (*, p<0.05 vs. no treatment ; **, p <0.05 vs. LPS alone; Ψ, p<0.05 vs. LPS+ Ghrelin).