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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Surgery. 2007 Dec 27;143(3):334–342. doi: 10.1016/j.surg.2007.09.039

Fig. 5. Exogenous ghrelin pretreatment augments LPS-induced release of IL-10 in a dose dependent and time-dependent manner through GHS-R in RAW 264.7 macrophages.

Fig. 5

The measurements are expressed in pg/ml units+/-SD and are derived from three independent experiments. Fig.5a shows that LPS provides effective stimulation for the release of IL-10 (*, p<0.05 vs. no treatment) and ghrelin pretreatment 4 h prior to LPS treatment (1μg/ml) for 24 h significantly stimulates release of IL-10 (**, p<0.05 vs. LPS alone). Fig.5b shows that ghrelin mediated accentuation of LPS-induced IL-10 release is time dependent being most pronounced at 24 h following LPS treatment (*, p<0.05 vs. LPS treatment alone). Fig 5c indicates that GHS-R antagonist D[lys-3]-GHRP-6 abrogates ghrelin-mediated augmentation of IL-10 release (*, p <0.05 vs. no treatment; **, p <0.05 vs. LPS alone; Ψ p<0.05 vs. LPS+Ghrelin).