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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Surgery. 2007 Dec 27;143(3):334–342. doi: 10.1016/j.surg.2007.09.039

Fig. 6. Exogenous ghrelin promotes enhanced activation of p38 MAPK in LPS-stimulated murine RAW264.7 cells.

Fig. 6

Western analysis; RAW 264.7 cells were pre-treated with peptides and chemicals for 1h before stimulation with LPS, cell lysates were harvested 15 min after LPS stimulation and were subjected to Western analysis for p-p38 MAPK, which was subsequently probed with p38 MAPK antibody to measure the total content of p38 MAPK; lane a, no treatment; lane b, LPS (1μg/ml); lane c, ghrelin (100nM) + LPS (1μg/ml); lane d, SB202190 (1μg/ml) + ghrelin (100nM) + LPS (1μg/ml); lane e; D[lys-3]-GHRP-6 (1μg/ml) + ghrelin (100nM) + LPS (1μg/ml).