TABLE 6.
Genotype | Daughter cell size (μm3) | Commitment size (μm3) | Doubling time (hr) |
---|---|---|---|
Wild type | 65 ± 2.7 | 200 ± 5 | 5.1 ± 0.3 |
smt7-1 | 65 ± 2.9 | ≥200a | 5.4 ± 0.3 |
smt14-1 | 66 ± 4.1 | 211 ± 11 | 5.3 ± 0.3 |
smt15-1 | 51 ± 4.1 | 159 ± 11 | 8.3 ± 0.5 |
smt16-1 | 66 ± 6.1 | 230 ± 11 | 5.3 ± 0.4 |
mat3-4 | 21 ± 1.4 | 103 ± 10 | 7.3 ± 0.2 |
smt7-1 mat3-4 | 37 ± 1.4 | 150 ± 22 | 6.8 ± 0.2 |
smt14-1 mat3-4 | 30 ± 1.6 | 151 ± 10 | 6.4 ± 0.2 |
smt15-1 mat3-4 | 37 ± 2.1 | 179 ± 13 | 9.7 ± 0.6 |
smt16-1 mat3-4 | 28 ± 1.2 | 126 ± 22 | 7.4 ± 0.3 |
Standard errors for doubling time, daughter cell size, and commitment size were derived from at least three independent cultures. For commitment size threshold determination standard errors were derived from multiple time points in each of three independent experiments.
The smt7-1 commitment size could not be accurately determined, but was at least as large as that of wild type. See results for details.