Figure 5.—

RNA profile of atg10-1 plants exposed to C-limiting conditions induced by extended darkness. Tissue was collected from wild-type (WT) and atg10-1 seedlings just after the indicated days of extended darkness (see Figure 3). Equal amounts of total RNA (10 μg) were subjected to gel-blot analysis using probes for ATG8a, ATG8e, CAB, SEN1, SAG12, PED1, GPX2, CSD1, CAT3, NYE1, and TUB4. Near equal loading of total RNA was confirmed by RNA gel-blot analysis of 18S rRNA (18S) and staining for total rRNA with methylene blue (data not shown).