TABLE 1.

Autophagic body accumulation in the vacuoles of wild-type and atg mutant seedlings

	+N−CA	−N−CA	+N+CA	−N+CA
Wild-type Col-0a	0.09 ± 0.01	0.05 ± 0.01	2.46 ± 0.07	3.88 ± 0.33
atg10-1b	0.08 ± 0.13	0.11 ± 0.14	0.03 ± 0.08	0.11 ± 0.18
atg5-1b	0.08 ± 0.13	0.08 ± 0.15	0.03 ± 0.08	0.08 ± 0.12
atg7-1b	0.07 ± 0.14	0.10 ± 0.13	0.13 ± 0.13	0.11 ± 0.13

Each line expressed GFP-ATG8 under the control of the CaMV 35S promoter. Seedlings were grown in N-deficient liquid medium for 2 days and then treated with 0.5 μm concanamycin A (CA) for 12–16 hr prior to confocal fluorescence microscopy of the central vacuole.

^aValues are the average number of vacuolar vesicles per 100 μm² of 9–32 cells each for three independent experiments (±SE).

^bValues are the average of number of vacuolar vesicles per 100 μm² of 12–36 cells each for one experiment (±SD).