Figure 9.—

LM–PCR analysis of resection reveals MRX-independent nucleotide loss. Wild-type, rad50, dnl4, and yku70 cells growing either exponentially (A) or arrested by α-factor (B) were induced to express HO with galactose and repressed with glucose 60 min later. LM–PCR was performed at various times as described in materials and methods using an oligonucleotide adapter capable of being ligated to an unresected HO-cut DSB. The signal from the LM–PCR product was expressed as a ratio relative to the AmpR control product and then normalized to the maximum value for each strain. LM–PCR signal persisted for a time and then disappeared in a manner independent of cell cycle stage, NHEJ, and MRX status.