Figure 1.—

sticky mutant cells have enlarged nuclei and increased ploidy. Follicle-cell layer of sti^Z3-5829/+ (A–C) and sti^Z3-5829 homozygotes (D–F) where DAPI-stained nuclei (blue) and cell membranes are stained with antiphosphotyrosine (P-tyrosine, green). (D) sti mutant cells have enlarged nuclei (asterisk) and multinucleated cells (arrows). Follicle-cell size and shape is also disrupted (E). Flow cytometry of DAPI-stained isolated nuclei from wild-type and sticky mutant ovaries: (G) sti^Z3-5829/+ follicle-cell nuclei terminate with normal 16c ploidy. (H) sti^Z3-5829 homozygous follicle cells have 32c ploidy. (I) Df(3L)eyg/+ follicles have normal ploidy, and (J) sti^Z3-5829/Df(3L)eyg have 32c ploidy. (K) sti³/+ follicle-cell nuclei have normal ploidy, and (L) sti^Z3-5829/sti³ have 32c ploidy. DAPI-stained larval brain mitotic cells of (M) wild-type sti^Z3-5829/+ and (N) sti^Z3-5829/Df(3L)ED4483 stained with DAPI. Follicle-cell images taken at ×40; bar, 10 μm. Mitotic chromosome images taken at ×100; bar, 1 μm.