Figure 2.—

Western blot analyses of Acp62F^1a–d null lines of whole male flies, dissected male accessory glands, and mated female flies. The solid arrow at the right marks Acp62F, which runs at ∼14 kDa (Lung and Wolfner 1999). Acp62F protein is produced in Acp62F^1a–d/TM3, Sb males and is not produced in Acp62F^1a–d/Acp62F^1a–d males (lanes 4–11). Acp62F is transferred in Canton-S females mated to Acp62F^1a/TM3, Sb males (lane 12), but not in Canton-S females mated to Acp62F^1a/Acp62F^1a males.