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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Mech Dev. 2007 Oct 5;125(1-2):117–129. doi: 10.1016/j.mod.2007.09.013

Fig. 1. Structure of Drosophila Follistatin.

Fig. 1

Like vertebrate FS proteins, dFS is subdivided into a N-terminal domain (N) and three FS domains (FS1-3). In contrast to its vertebrate orthologues, dFS encodes an unusually long signal peptide trailer, which contains three potential transmembrane domains (84-104, 161-180, 194-211), and a 260 amino acid insertion in FS1, which adds to the overall positive charge of the protein. In this study, we use a truncated cDNA that encodes a shorter signal leader that starts after amino acid 62. The location of the HA and RGS-6His tags in the constructs used in this study are indicated on top. In the absence of protein modifications, the predicted sizes of the signal leader and the processed full-length secreted form are 16 kD and 62 kD respectively.