Fig. 4. Effect of GFP-tagged wild-type and kinase-dead c-Abl on H₂O₂-NOX5 regulation.

Superoxide production was determined in the presence or absence of 100 μM H₂O₂. (A) K562 cells stably expressing GFP-c-Abl or GFP-KD-c-Abl were transfected with the pRep4 vector encoding the NOX5 protein. (B) HEK/NOX5 cells transiently transfected with either an empty vector or the wild-type c-Abl or kinase-dead c-Abl. Superoxide production was expressed as a percent of the value obtained for non-treated cells in the absence of H₂O₂. The data are the means +/− SEM of 3 to 4 independent experiments. Asterisks indicate statistical significance versus control cells without H₂O₂ * P < 0.05; ** P <0.01, Numbers above the first column of each graph represent the mean +/− SEM of the chemiluminescence output observed in those samples at 10 minutes.