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. 2008 Mar 20;105(13):5242–5247. doi: 10.1073/pnas.0801197105

Fig. 3.

Fig. 3.

History of mammary tumors is associated with tumor recurrence and reduced survival. (A) Time of appearance of recurrent tumors in a cohort of mature female TOM;TOR;MTB mice exposed to doxycycline from 4 to 20 weeks of age (median, 8 weeks) until tumor burden reached an estimated 10% of body weight and then placed on a doxycycline-free diet (dashed-dot line; n = 36), compared with time of appearance of primary tumors in TOM;TOR;MTB control female mice never exposed to doxycycline (solid line; n = 31). (B) Time of appearance of recurrent tumors in a cohort of mature female Myc;TOR;MTB mice exposed to doxycycline from 4 to 20 weeks of age (median, 9 weeks) until total tumor burden reached an estimated 10% of body weight and then placed on a doxycycline-free diet (dashed-dot line; n = 14) compared with time of appearance of primary tumors in bitransgenic Myc;TOR control female mice on doxycycline (solid line; n = 22). (C) Survival of animals after doxycycline removal in TOM;Kras;MTB mice (solid line; n = 12), Myc;TOR;MTB (dashed-dot line; n = 12), and TOM;TOR;MTB (dotted line, n = 36). The animals were removed from doxycycline when the initial tumor burden reached an estimated 10% of body weight. Mice were euthanized when total recurrent tumor burden reached an estimated 10% of body weight, and these time points were used to calculate survival time after doxycycline withdrawal.