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. 1998 Jun 9;95(12):7203–7208. doi: 10.1073/pnas.95.12.7203

Figure 1.

Figure 1

(A) Plasmid pWRG 2426 contains hpt as the selectable gene flanked by two nonselected genes, bar and gusA. All three genes are driven by CaMV35S promoter. The bar and hpt are transcribed in one direction and the gusA in the opposite direction. The bar gene has the maize Adh1 5′ intron between the promoter and coding sequence, and bar and hpt have the nosA terminator but gusA has the ssuA terminator. Restriction enzymes XbaI and SacI separate one gene from the other two as shown by the horizontal bars. This feature of the construct is particularly useful in determining the transgene organization after integration into the genome. By using forward primers for bar (BaF1) and gusA (GusF1) a 5.6-kb fragment is amplified through long PCR. (B) Plasmid pWRG 4517 contains hpt as the selectable gene and bar as the nonselected gene; both genes have CaMV35S promoter and SoypA terminator and transcribe in opposite directions. Restriction enzymes SacI and XbaI both separate the two genes from each other. By using forward primers for bar (BarF1) and hpt (HptF1) a 2.4-kb fragment is amplified through PCR.