Figure 2.

(A) Digestion of genomic DNA of 15 transgenic lines with restriction enzyme NheI and subsequent Southern hybridization with the hpt coding region as probe results in a single band for 11 lines. As NheI does not have a restriction site on the construct this indicates integration of the transgenes at the single locus. The four plant lines K496-3 (lane 3), K496-4 (lane 4), B550-9 (lane 8), and G517-3 (lane 12) show more than one band. However, these lines result in a single band with other restriction enzymes that do not cleave within the construct (see Results) as exemplified by (B) where genomic DNA of K496-2 was digested with restriction enzymes ClaI (lane 1), NheI (lane 2), BstXI (lane 3), and SfiI (lane 4) and probed with the coding region of gusA. Only BstXI results in two bands.