Table 1.
Determination of copy number of transgenes for transformants of pWRG 2426
| Plant line | Copy number estimation with XbaI
|
Copy number estimation with SacI
|
Copy number determination using transgene organization
|
Total number of transgenes*
|
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| bar | hpt | gus | bar | hpt | gus | bar | hpt | gus | bar + hpt + gus | |
| K495-1 | 3 | 3 | 3 | 3 | 3 | 4 | 3 | 3 | 4 | 10 |
| K496-2 | 1 | 2 | 2 | 2 | 3 | 3 | 2 | 3 | 3 | 8 |
| K496-3 | 2 | 5 | 4 | 5 | 5 | 6 | 4 | 5 | 6 | 15 |
| K496-4 | 4 | 4 | 4 | 5 | 4 | 5 | 5 | 4 | 5 | 14 |
| C549-1 | 2 | 1 | 1 | 2 | 1 | 1 | 2 | 1 | 1 | 4 |
| I533-1 | 3 | 3 | 3 | 3 | 2 | 3 | 3 | 3 | 3 | 9 |
| I533-3 | 3 | 2 | 3 | 3 | 2 | 3 | 3 | 2 | 3 | 8 |
| B550-9 | 5 | 4 | 4 | 5 | 3 | 3 | 6 | 4 | 4 | 14 |
| L551-4 | 2 | 3 | 2 | 2 | 2 | 2 | 2 | 3 | 2 | 7 |
| L551-8 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 6 |
| L551-10 | 3 | 4 | 3 | 3 | 4 | 3 | 3 | 4 | 3 | 10 |
*Based on transgene organization.