Skip to main content
. 2008 Mar 24;105(13):5189–5194. doi: 10.1073/pnas.0706296105

Fig. 2.

Fig. 2.

Four days of antigenic stimulation is necessary for optimal for CD8-T cell induction. C57BL/6 mice were immunized with fixed cumulative dose of 125 μg of gp33 peptide and 12.5 nmol of CpG according to different schedules (A) with peaks at day 0 (bolus), day 3, day 5, or day 7. At different time points thereafter, mice were bled, and the CD44 expression and the IFN-γ secretion of CD8 T cells after restimulation in vitro with gp33 peptide were analyzed (B and C). The FACS density blots illustrate the frequencies of CD44hi and IFN-γ-producing CD8-positive lymphocytes as measured by FACS at the peak of the immune response, and the numbers show the mean percentage of IFN-γ-producing CD44hi CD8+ T cells. The mean percentage of IFN-γ-producing CD44hi CD8+ T cells is also illustrated as a function of time (C). One of two similar experiments is shown (n = 3–4).