Fig. 5.

Association of ADAR1 and hUpf1 is revealed by reversible cross-linking and analysis on 2D gels. HeLa cell NE was cross-linked by the reversible cross-linker DTBP for 20 min. (A) First-dimension SDS/PAGE analysis of untreated NE (−) and cross-linked NE (+). (B and C) The gel was soaked in 3% β-mercaptoethanol and electrophoresed on a second identical gel. Western blotting was performed with anti-ADAR1 and anti-hUpf1 as marked on the right. (D) A merge of B and C. The arrows mark the direction of migration of the first and second dimension of the gel.