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. 2008 Mar 24;105(13):5028–5033. doi: 10.1073/pnas.0710576105

Fig. 6.

Fig. 6.

Up-regulation of MGC10471, DAP3, PISD, and SARS mRNAs by RNAi of ADAR1. HeLa cells were either transfected with siRNA against ADAR1, siRNA against firefly luciferase as a negative control or not transfected at all. After 72 h, total RNA and total proteins were prepared. (A) Western blot analyses of a total protein preparation extracted from the above treated cells show specific down-regulation of ADAR1 (≈20%). (B) (Right) RT-PCR analyses of actin, ADAR1, MGC10471, DAP3, PISD, SARS, MAP3K14, TXNRD2, SDHA, and hUpf1 RNAs extracted from HeLa cells treated with siRNA against ADAR1 (lane 1), luciferase (lane 2), and from untreated cells (lane 3). (Left) The image represents 3-fold serial dilutions of RNA from untreated cells to demonstrate that the RT-PCR is semiquantitative.