Figure 8. Sensitivity and specificity of fusion gene predictions.

(A) Number of false positive (FP) and true positive (TP) fusion gene predictions for a simulated genome with 100 translocations and 10,000 paired reads. Each curve represents the average of 50 simulations with clones of a fixed length (2 kb, 40 kb, 150 kb clones). The minimum fusion probability threshold for indicating that a fusion gene was predicted was decreased from >.95 (leftmost point) to >0 (rightmost point) in increments 0.05 and the number of true and false predictions was determined. For all figures 19 true fusion genes were present in the rearranged genome. These 19 events were not selected for but rather they resulted from random rearrangement of the genome. (B) 100,000 paired reads. (C) 1,000,000 paired reads. (D) 10,000,000 paired reads.