Figure 3.
Effect of DOPC on COX-1 activity. COX-1 (15 nM) was assayed at 23 °C in reactions with 10 μM arachidonate and the indicated level of DOPC. The enzyme activity was determined from the extent of oxygen consumption after 20 s of reaction and was normalized to the control without DOPC. The line represents the fit to a two-parameter logistic equation, v = 1 / (1 + ([DOPC] / IC50)n) (38).