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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Prostaglandins Other Lipid Mediat. 2007 Dec 8;85(3-4):134–143. doi: 10.1016/j.prostaglandins.2007.12.001

Figure 5.

Figure 5

Effect of temperature on COX-1 inhibition by DMPC. Reaction mixtures contained 10 μM arachidonate and 0−833 μM DMPC at indicated temperature. Each reaction was started by injection of 16 nM PGHS-1. The COX activity, expressed as a fraction of the control, was calculated from oxygen consumption at the time the control reaction reached 50% completion. The data for fractional activity vs. DMPC concentration at each temperature were fitted to a two-parameter logistic equation, v = 1 / (1 + ([DMPC] / IC50)n) to estimate the IC50 value and the standard error of the fit (38).