Skip to main content
. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Prostaglandins Other Lipid Mediat. 2007 Dec 8;85(3-4):134–143. doi: 10.1016/j.prostaglandins.2007.12.001

Figure 7.

Figure 7

Comparison of COX-1 kinetics in microsomal PGHS-1 before and after membrane disruption with detergent. The COX-1 velocity was determined at the indicated arachidonate levels for membrane bound PGHS-1 in sheep seminal vesicle microsomes and for the enzyme solubilized by addition of 0.9 % (v/v) Tween 20 to the same microsomes. The reaction buffer was 0.1 M Tris, pH 8.5, containing 0.1 mM phenol and 1 μM heme, and was thermostatted at 23 °C. Dilution of the detergent treated sample by the reaction mixture (1:375) was used to minimize the effect of the detergent itself. The activity was normalized to the protein concentration; each point represents the average of duplicate reactions. The data were fitted to the Michaelis-Menten equation to estimate values for Vmax and Km shown in the insert (± the SD for the fit).