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A mechanical release-restretch manoeuvre was used to measure the rate of force redevelopment in a solution of pCa 9.0 in the absence and presence of 6 μm NEM-S1. This skinned myocardial preparation generated Ca2+-independent force of 0.05 Po in the absence of NEM-S1 in pCa 9.0 (trace a), whereas Ca2+-independent force increased to 0.22 Po following NEM-S1 treatment (trace b).
