Figure 7. DEANO does not inhibit the stimulation of I_Ca,L induced by exogenous cAMP.

A, the pipette solution contained 30 μM cAMP, and cAMP dialysis started when the patch was ruptured (arrow). B, a myocyte was first dialysed with the control solution, and intracellular dialysis with 30 μM cAMP started at the time indicated by the arrow. Applications of DEANO (100 μM) in A or SNP (5, 50 and 500 μM) in B were performed as indicated by the horizontal lines. In A, current traces on top were recorded at the times indicated by the corresponding letters on the main graph. The dotted line indicates the zero-current level. C, summary of the effects of DEANO, SNP and SNAP on the cAMP (10–100 μM)-stimulated I_Ca,L. Variations are given as percentage change over the amplitude of the cAMP-stimulated I_Ca,L. Bars are the means and lines are the s.e.m. of the number of experiments indicated near the bars.