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Cells were superfused with Krebs solution (2 mm Ca2+). Agonists (in Ca2+-free solution) were applied as indicated by the filled bars. Repetitive applications of 50 μm carbachol (A) or 100 μm ATP (B) evoked reproducible [Ca2+]i elevations (lower traces) and [Ca2+]L decreases (upper traces). Left-hand axes, [Ca2+]i; right-hand axes, SR [Ca2+]L.
