Figure 11. Conditioning pre-pulses do not facilitate cAMP-potentiated L-channels.
L-channel activity recorded before and after a conditioning pre-pulse (+150 mV, 280 ms) in control patches pre-incubated with 1 mm 8-CPT-cAMP for 30 min. Repolarisations to -80 mV lasted 40 ms. Most traces displayed high-Po activity before the conditioning pulse. Pre-pulses were ineffective in either accelerating the activation kinetics or increasing the Po of L-channels. The mean currents below were calculated over 55 sweeps pooled from 10 patches. The bottom traces were obtained from 37 sweeps pooled from 4 patches pre-incubated with 8-CPT-cAMP and with the agonists in the pipette.