Figure 6. Effect of 20 μm TBQ on SR Ca²⁺ leak.

Preparations were loaded under standard conditions and then leaked for 2 min in Leak₂ solution containing either 1.04 mm ADP or 1.04 mm ADP plus 20 μm TBQ (see Methods). Both responses are compared to Ca²⁺ remaining in SR after standard loading conditions (2 min Load₃ solution, pCa 6.7) and were found to be significantly different (P < 0.05, Student's paired t test, n= 3).