Table 1.
Composition of standard and modified Krebs solutions
| Ca2+ -containing solutions | Ca2+ -free solutions | |||||
|---|---|---|---|---|---|---|
| 2Ca-5K | 2Ca-100K | 2Ca-150K | 0Ca-5K | 0Ca-100K | 0Ca-150K | |
| Na+ | 146.0 | 51.0 | 1.0 | 150.0 | 55.0 | 5.0 |
| K+ | 5.0 | 100.0 | 150.0 | 5.0 | 100.0 | 150.0 |
| Ca2+ | 2.0 | 2.0 | 2.0 | 0 | 0 | 0 |
| Mg2+ | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 |
| Cl− | 131.0 | 6.55 | 4.37 | 131.0 | 6.55 | 4.37 |
| MS−* | 0 | 124.45 | 126.63 | 0 | 124.45 | 126.63 |
| HCO3minus; | 25.0 | 25.0 | 25.0 | 25.0 | 25.0 | 25.0 |
| H2PO4− | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 |
| Glucose | 10.0 | 10.0 | 10.0 | 10.0 | 10.0 | 10.0 |
Concentrations are given in mm. The product of [K+] × [Cl−] was kept constant in the different solutions. All the solutions were saturated with 95% O2−5% CO2.
*
MS−, methanesulfonate.