Table 2.

Rates of Mn²⁺ quench before and after depletion of store Ca²⁺

Depletion procedure		n	Before depletion (% FI min−1)	After depletion (% FI min−1)	After refilling of Ca2+ (% FI min−1)
Without SERCA inhibitor		13	6.0 ± 3.0	9.6 ± 1.2	n.d.
With SERCA inhibitor	CPA, 10 μm	8	5.5 ± 1.2	37.8 ± 4.8**	6.0 ± 1.8
	BHQ, 10–30μm	4	5.4 ± 1.2	12.0 ± 3.6	9.0 ± 3.0
	Thapsigargin, 10 μm	4	6.4 ± 0.6	36.2 ± 7.2 *	7.9 ± 1.7

The decline of the fura-2 fluorescence intensity is expressed as percentage per minute (initial fluorescence intensity = 100%). Data are means ±s.e.m.; n, number of determinations. n.d., not determined.

^*P < 0.05

^**P < 0.01.