Figure 5. Mitochondrial [Ca²⁺] and [NADH] as measured in pBC cells during hypoxia.

The cells were stained with Rhod-2 to monitor [Ca²⁺]_m changes and the fluorescence signals were collected as described in Methods. The results for two representative cells are shown, one with and one without [Ca²⁺]_m and NADH signal oscillations, which correlated with respiratory (XII) output. The data at the time marked by an asterisk on the left are presented on an expanded time scale on the right.