Skip to main content
. 1998 Jun 9;95(12):7225–7230. doi: 10.1073/pnas.95.12.7225

Figure 2.

Figure 2

The 48-kDa kinase activated by SA and fungal cell wall elicitor corresponds to SIPK. (A) Protein extracts (50 μg) from water-infiltrated (0 and 180 min) and SA-infiltrated (0 and 180 min) leaves were immunoprecipitated with the SIPK-specific antibody Ab-p48N in the absence or presence of a competitor peptide (either p48N or p44N). Kinase activity of the immune-complex subsequently was determined with an in-solution kinase assay by using MBP as the substrate, and the phosphorylated MBP was visualized by autoradiography after SDS/PAGE. (B) Protein extracts (50 μg) from water-infiltrated (0 and 30 min) and fungal cell wall elicitor (Eli)-infiltrated (0 and 30 min) leaves were immunoprecipitated with antibody Ab-p48N in the absence or presence of a competitor peptide. Kinase activity of the immune-complex subsequently was determined as described in A.