Figure 7.

Water infiltration and wounding induce transient increases in WIPK mRNA levels but little or no increases in WIPK protein level. (A) Total RNA was extracted at the indicated times from water-infiltrated or wounded leaves and was subjected to RNA blot analysis. Blots were hybridized sequentially with the 3′ untranslated region and then the full length WIPK cDNA. Both probes yielded the same result; thus, only the autoradiogram produced with the full-length cDNA probe is shown. (B) Protein extracts (20 μg) were subjected to immunoblot analysis with the WIPK-specific antibody Ab-p44N.