Figure 5. Filipin depletes rat type II alveolar epithelial cells of caveolin-1-positive, albumin-containing vesicles, but not lysosomes.

Control or filipin-treated (10 nm, 30 min) cells were stained (37 °C, 30 min) with LysoTracker (50 nm) plus Alexa 488-labelled albumin (50 μg ml⁻¹), fixed and immunostained with anti-caveolin-1 mAb plus goat-anti-mouse Alexa 350-conjugated secondary Ab. Control cells received only the filipin vehicle DMSO. Results are representative of 3 experiments. Scale bar, 5 μm in all panels.