Figure 2. Confocal micrographs of 8 μm cryosections, showing double labelling with Kv1.1 (FITC) and vimentin (Texas red) antibodies at the submucosal-circular muscle interface (SCMI) of canine proximal colon.

A, Kv1.1-like immunoreactivity (Kv1.1-LI, green), showing intense staining (arrow) at the submucosal-circular muscle interface (SCMI) between the circular muscularis (CM) and submucosa (SM). A network of ICC is found at this level and is positive for Kv1.1. B, vimentin-like immunoreactivity (vimentin-LI, red) of the same specimen, showing labelling of ICC in the SCMI and neurons (arrowheads) in the circular muscularis. C, co-localization (yellow) of Kv1.1-LI and vimentin-LI. Negative controls where primary antibodies were omitted showed no staining.