Figure 1. Transcript analysis of cardiac tissue.

Each lane was loaded with 25 μg of total neonatal ventricular RNA from wild-type (WT), Kir2.1^−/− or Kir2.2^−/− mice and probed with a portion of the open reading frame of Kir2.1, Kir2.2 or Kir2.3. Kir2.1 and 2.3 are each encoded by a single transcript while Kir2.2 is encoded by two. Equal loading of RNA was confirmed by re-probing the blots with a fragment of the GAPDH open reading frame.