Figure 4. Immunoblot of inducible nitric oxide synthase in human umbilical artery smooth muscle cells after stimulation with insulin.

Smooth muscle cells isolated from non-diabetic or gestational diabetic pregnancies were deprived of serum for 24 h, washed and incubated with culture medium in the absence (-) or in the presence (+) of insulin (8 h). Data are representative of similar blots obtained in 3 different cell cultures.