Table 2.
Kd | Bmax | |
---|---|---|
Non-diabetic | ||
Control | 0.16 ± 0.03 | 0.66 ± 0.07 |
l-NAME | 0.12 ± 0.02 | 0.55 ± 0.01 |
Cycloheximide | 0.12 ± 0.02 | 0.7 ± 0.02 |
SQ-22536 | 0.14 ± 0.03 | 1.3 ± 0.02* |
Insulin | 0.15 ± 0.01 | 1.1 ± 0.1* |
Insulin +l-NAME | 0.17 ± 0.03 | 0.71 ± 0.09† |
Insulin + cycloheximide | 0.17 ± 0.03 | 0.64 ± 0.04† |
Insulin + SQ-22563 | 0.16 ± 0.01 | 1.1 ± 0.01* |
Diabetic | ||
Control | 0.15 ± 0.03 | 1.62 ± 0.16* |
l-NAME | 0.12 ± 0.02 | 0.75 ± 0.11‡ |
Cycloheximide | 0.13 ± 0.01 | 1.2 ± 0.1* |
SQ-22536 | 0.14 ± 0.01 | 1.5 ± 0.09* |
Insulin | 0.18 ± 0.01 | 0.6 ± 0.13‡ |
Insulin +l-NAME | 0.10 ± 0.03 | 0.75 ± 0.09*§ |
Insulin + cycloheximide | 0.11 ± 0.03 | 0.59 ± 0.03‡ |
Insulin + SQ-22536 | 0.13 ± 0.01 | 1.50 ± 0.2*§ |
Kinetic parameters for equilibrative [3H]NBMPR binding (30 min, 22°C) were determined in smooth muscle cells incubated in medium 199 in the absence (Control) or in the presence of insulin (1nm, 8 h), NG-nitro-L-arginine methylester (l-NAME, 100 μm, 30 min), cycloheximide (1μm, 8 h) or SQ-22536 (100 μm, 30 min), and binding was assayed in Krebs solution. Values for Kd and Bmax are in nm and fmol (μg protein)−1, respectively, and are the means ±s.e.m. of experiments in 3–4 different cell cultures;
P < 0.05 vs. corresponding control values in non-diabetic cells,
P< P < 0.05 vs. values in non-diabetic cells treated with insulin,
P< P < 0.05 vs. corresponding control values in non-diabetic cells,
P < 0.05 vs. values in diabetic cells treated with insulin.