Figure 4. SPNK1 potentiation of XII nerve inspiratory burst amplitude is reduced by neurokinin receptor antagonists.
A, effects of 0.1 μm SPNK1 locally applied for 10 s on integrated XII nerve activity before (control) and after 1 and 3 h of incubation with spantide (5 μm). B, effects 0.1 μm SPNK1 locally applied for 10 s before (control) during and 15 min after (washout) a 5 min pre-application of 10 μm GR-82334 locally over the XII nucleus. C, mean (±s.e.m.) inhibition by spantide (5 μm, bath applied, n = 5) or GR82334 (10 μm, locally applied, n = 8) of the SPNK1-mediated potentiation of inspiratory burst amplitude (0.1 μm SPNK1, 10 s local application). * Significant increase from control. Note, spantide and GR82334 were tested in different preparations.