Table 1.

Effects of 11,12-DHET on the open and closed time constants of BK channels in rat coronary vascular smooth muscle cells

	Control	1 nM DHET	10 nM DHET	100 nM DHET
τo,1(ms)	0.36 ± 0.02	0.50 ± 0.02*	0.56 ± 0.02*	0.93 ± 0.02*
(Ao,1)	(0.16 ± 0.04)	(0.21 ± 0.05)	(0.33 ± 0.06)*	(0.45 ± 0.15)*
τo,2(ms)	2.06 ± 0.55	2.74 ± 0.42	3.64 ± 0.40	7.30 ± 0.32*
(Ao,2)	(0.24 ± 0.10)	(0.50 ± 0.14)	(0.57 ± 0.13)	(0.49 ± 0.14)
τo,3(ms)	10.9 ± 2.5	12.6 ± 5.8	43.1 ± 9.2*	126.3 ± 32.4*
(Ao,3)	(0.60 ± 0.12)	(0.32 ± 0.16)	(0.16 ± 0.15)*	(0.08 ± 0.06)*
τc,1(ms)	0.48 ± 0.05	0.45 ± 0.05	0.34 ± 0.07	0.20 ± 0.07*
(Ac,1)	(0.15 ± 0.03)	(0.16 ± 0.05)	(0.25 ± 0.04)*	(0.32 ± 0.02)*
τc,2(ms)	2.79 ± 0.35	2.33 ± 0.51	1.70 ± 0.30	1.24 ± 0.45
(Ac,2)	(0.31 ± 0.06)	(0.35 ± 0.07)	(0.35 ± 0.05)	(0.38 ± 0.01)
τc,3(ms)	7.69 ± 1.53	6.41 ± 1.57	6.33 ± 1.52	4.56 ± 1.10
(Ac,3)	(0.32 ± 0.04)	(0.28 ± 0.08)	(0.31 ± 0.07)	(0.30 ± 0.08)
τc,4(ms)	495.2 ± 55.1	245.2 ± 79.0	195.2 ± 73.3*	76.9 ± 6.7*
(Ac,4)	(0.21 ± 0.08)	(0.14 ± 0.07)	(0.10 ± 0.04)	(0.01 ± 0.01)

Data represent the mean ± S.E.M., n = 4

^*P < 0.05 vs. control. The time constants (τ) and the relative areas or weights (A, in parentheses) are presented. τ_o,1, τ_o,2 and τ_o,3 represent the fast, intermediate and slow components of the BK channel open times and A_o,1, A_o,2 and A_o,3 represent the relative weights of τ_o,1, τ_o,2 and τ_o,3, respectively, where A_o,1 + A_o,2 + A_o,3 = 1. τ_c,1, τ_c,2, τ_c,3and τ_c,4 represent the fast, intermediate, slow and very slow components of the BK channel closed times and A_o,1, A_o,2, A_o,3 and A_o,4 represent the relative weights of τ_c,1, τ_c,2, τ_c,3 and τ_c,4, respectively, where A_c,1 + A_c,2 + A_c,3 + A_c,4 = 1.