Figure 2. Cannabinoids inhibit glycinergic eIPSCs in medullary dorsal horn neurons.

A, time course of eIPSC amplitude, before and during application of WIN55,212-2 (WIN, 3 μm), and after addition of SR141716A (SR, 3 μm) and strychnine (5 μm). B, averaged eIPSCs from the cell in A, showing amplitude size before (Control) and during application of WIN55,212-2 (3 μm), and after application of SR141716A (3 μm). C, normalized average responses to identical paired stimuli (inter-stimulus interval, 70 ms), with eIPSC₁ normalized (left) to demonstrate the facilitation of eIPSC₂ relative to eIPSC₁ during application of WIN55,212-2, and its recovery after application of SR141716A. Neurons were voltage clamped at -74 mV, and IPSCs evoked at 15 s intervals in the presence of APV (20 μm), bicuculline (30 μm) and CNQX (5 μm).