Table 2.
Captopril (50 μm) |
Spironolactone (100 μm) |
Aldosterone (osmotic pump) | |||||
---|---|---|---|---|---|---|---|
HS | LS | HS | LS | HS | LS | HS | |
Jejunum | |||||||
Vmax | 1525 ± 32 | 1602 ± 101 | 1498 ± 44 | 1563 ± 12 | 1626 ± 74 | 1702 ± 106 | 1679 ± 34 |
B1 | 53 ± 6 | 56 ± 5 | 60 ± 6 | 62 ± 5 | 59 ± 4 | 60 ± 4 | 58 ± 2 |
Ileum | |||||||
Vmax | 1725 ± 124 | 1234 ± 131 * | 1530 ± 106 | 1627 ± 98 | 1714 ± 34 | 1736 ± 50 | 1194 ± 70 * |
B1 | 49 ± 1 | 30 ± 2 * | 49 ± 3 | 53 ± 3 | 51 ± 5 | 50 ± 3 | 30 ± 6 * |
Rectum | |||||||
Vmax | 1206 ± 75 | 728 ± 40 * | 1234 ± 65 | 1178 ± 115 | 1197 ± 102 | 1303 ± 28 | 692 ± 24 * |
B1 | 36 ± 2 | 14 ± 1 * | 35 ± 4 | 36 ± 4 | 30 ± 2 | 35 ± 4 | 15 ± 3 * |
D-Glc Vmax is expressed as pmol (mg protein)−1 s−1 and the specific cytochalasin B binding, determined using 1 μM cytochalasin B (B1), is expressed as pmol cytochalasin B (mg protein)−1. Values are the means ± s.e.m. of 3 separate experiments.
P < 0.05 compared with HS. There were no significant differences between Km and Kd values (see Results).