Table 1.

Solutions used for whole-cell patch-clamp recordings

	CaCl2	MgCl2	NaCl	KCl	NaH2PO4	NaHCO3	Glucose	pH
Ext 1	0.5–5	2	125–117.5	3	1.2	26	10	7.4
Ext 2	0.5–4.5	4.5–0.5	122	3	1.2	26	10	7.4
	CaCl2	MgCl2	NaCl	TEA	Hepes	Choline chloride	Glucose	pH
Ext 3	0.5–5	1	—	20	10	100.5–105	10	7.4
	CaCl2	MgCl2	NaCl	KCl	Hepes	Choline chloride	Glucose	pH
Ext 4	4.5–0	0–4.5	141	3	10	—	10	7.4
Ext 5	0.01–0.1	—	141	3	10	—	10	7.4
	0.5–1–5
	10
Ext 6	4.5–0	0–4.5	—	—	10	144	1	7.4
Ext 7	4.5–0	0–4.5	—	20	10	124	10	7.4
Ext 8	4.5–0	0–4.5	—	144	10	—	10	7.4
Ext 9	4.5–0	0–4.5	20	—	10	124	10	7.4
Ext 10	4.5–0	0–4.5	144	—	10	—	10	7.4
Ext 11	0.5–4.5	—	141	3	10	—	10	7.4
Ext 12	—	0.5–4.5	141	3	10	—	10	7.4
Ext 13	0.5–4.5	4.5–0.5	141	3	10	—	10	7.4
	CaCl2	MgCl2	Potassium gluconate	KCl	Hepes	EGTA	—	pH
Int 1	1	1	135	—	10	11	—	7.3
Int 2	1	2	—	140	10	11	—	7.3
	CsCl	MgCl2	TEA	—	Hepes	EGTA	Glucose	pH
Int 3	110	0.8	30	—	10	10	8	7.3

Ext, extracellular solution; Int, intracellular solution. Concentrations are millimolar. Solutions Ext 3, 6, 7 and 8 and Int 3 were titrated with CsOH.