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. 2001 Sep 1;535(Pt 2):553–564. doi: 10.1111/j.1469-7793.2001.t01-1-00553.x

Figure 1. Relationship between amount of samples and density of Western blots for CPI-17 and MYPT1.

Figure 1

A shows a representative Western blot using polyclonal anti-CPI-17 antibodies against purified recombinant CPI-17; B shows a blot using monoclonal anti-MYPT1 antibodies against a control sample extracted from VD (n = 3 for both). The Western blots were prepared with 0-45 ng of purified protein for CPI-17 and 0-45 μg of total VD protein for MYPT1. Values for no protein were indiscernible from background values.